Volume 2, Issue 1 (Winter 2016)                   Caspian.J.Neurol.Sci 2016, 2(1): 1-8 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Farjah G, Naeimi M, Saberi A. Comparison Outcome of Nerve Regeneration across an Eggshell Membrane Guidance Channel and Autograft . Caspian.J.Neurol.Sci 2016; 2 (1) :1-8
URL: http://cjns.gums.ac.ir/article-1-80-en.html
1- Neurophysiology Research Center, Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran ; hfarjah@hotmail.com
2- Resident of Emergency Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3- Graduated from School of Medicine, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Abstract:   (4767 Views)

Background: Peripheral nerves may be damaged during an injury and its current standard treatment is using an autologous nerve.

Objectives: The purpose of this experimental study is to evaluate and compare the histological results of nerve regeneration after using the eggshell membrane (ESM) guidance channel with autograft.

Materials and Methods: Thirty adult male rats were divided into three experimental groups: ESM guidance channel, autograft, and sham surgery. The decalcifying membrane of egg rotated over the Teflon mandrel and dried at 37°C. A 10 mm nerve segment of left sciatic nerve was cut and removed. In ESM group, the ends of the sciatic nerve were telescoped into the nerve guides. In autograft group, the nerve segment was reversed and used as an autologous nerve graft. At 90 days after surgery, all animals were evaluated by histological and immunohistochemical assessment.

Results: The diameters of regenerated myelinated fibers were 5.24±2.14 µm for the ESM group, and 5.89±2.99 µm for the autograft group. The number of myelinated axons regenerated in the ESM group (9824±218 nerve fibers) was significantly greater than autograft group (7865±314 nerve fibers) (p<0.05).

Conclusion: These findings demonstrate that ESM effectively enhances nerve regeneration in injured rat sciatic nerve.

Full-Text [PDF 842 kb]   (1992 Downloads) |   |   Full-Text (HTML)  (2014 Views)  
Type of Study: Research | Subject: Special
Received: 2016/03/6 | Accepted: 2016/03/6 | Published: 2016/03/6

Add your comments about this article : Your username or Email:

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Caspian Journal of Neurological Sciences

Designed & Developed by : Yektaweb