پنجشنبه 6 اردیبهشت 1403
دوره 8، شماره 4 - ( 6-1401 )
جلد 8 شماره 4 صفحات 212-206 |
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Hashemi M, Karami M, Jafarpour Fard M. Effect of Sulpiride on Dopaminergic Synapse of Dorsal Hippocampus of Morphine-Treated Rats. Caspian J Neurol Sci 2022; 8 (4) :206-212
URL: http://cjns.gums.ac.ir/article-1-563-fa.html
URL: http://cjns.gums.ac.ir/article-1-563-fa.html
Effect of Sulpiride on Dopaminergic Synapse of Dorsal Hippocampus of Morphine-Treated Rats. مجله علوم اعصاب کاسپین. 1401; 8 (4) :206-212
چکیده: (881 مشاهده)
Background: As previous studies show, several effects of morphine are induced by the dopaminergic
system. Sulpiride is a dopamine D2 receptor (DAD2) antagonist widely used in clinics to treat DArelated
disorders. DAD2 receptors are abundant at hippocampal cornu ammonis (CA1).
Objectives: This study aimed to investigate the possible interaction of morphine and sulpiride on
DA synapses in CA1.
Materials & Methods: In this study, 48 Wistar rats weighing 220 to 250 g were used. These animals
were classified into eight groups (6 rats per group): saline control group (1 ml/kg), morphine
group (5 mg/kg), sulpiride groups alone (1, 2, and 4 mg/kg) and sulpiride groups (1, 2, and 4 mg/
kg)+morphine (5 mg/kg). Saline or substances were injected once intraperitoneally. After 48 h, the
animals’ brains were removed under anesthesia and placed in 10% formalin for fixation. Then, 3- to
4-μm slices were cut from these tissues, and the DA synapse was examined by histochemistry and
immunohistochemistry techniques. The data were statistically analyzed by the analysis of variance.
Results: The control group had DA synapses and healthy neurons. A relative increase in DA
synapses compared to the control group was observed in the morphine and single sulpiride groups.
However, in sulpiride+morphine groups, DA synapses were reduced compared to morphine or
sulpiride alone, but neurons were not destroyed.
Conclusion: The interaction effect of sulpiride and morphine in the CA1 region may decrease DA
synapses.
system. Sulpiride is a dopamine D2 receptor (DAD2) antagonist widely used in clinics to treat DArelated
disorders. DAD2 receptors are abundant at hippocampal cornu ammonis (CA1).
Objectives: This study aimed to investigate the possible interaction of morphine and sulpiride on
DA synapses in CA1.
Materials & Methods: In this study, 48 Wistar rats weighing 220 to 250 g were used. These animals
were classified into eight groups (6 rats per group): saline control group (1 ml/kg), morphine
group (5 mg/kg), sulpiride groups alone (1, 2, and 4 mg/kg) and sulpiride groups (1, 2, and 4 mg/
kg)+morphine (5 mg/kg). Saline or substances were injected once intraperitoneally. After 48 h, the
animals’ brains were removed under anesthesia and placed in 10% formalin for fixation. Then, 3- to
4-μm slices were cut from these tissues, and the DA synapse was examined by histochemistry and
immunohistochemistry techniques. The data were statistically analyzed by the analysis of variance.
Results: The control group had DA synapses and healthy neurons. A relative increase in DA
synapses compared to the control group was observed in the morphine and single sulpiride groups.
However, in sulpiride+morphine groups, DA synapses were reduced compared to morphine or
sulpiride alone, but neurons were not destroyed.
Conclusion: The interaction effect of sulpiride and morphine in the CA1 region may decrease DA
synapses.
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