Volume 8, Issue 4 (Autumn 2022)
Caspian J Neurol Sci 2022, 8(4): 206-212 |
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1- Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
Abstract: (981 Views)
Background: As previous studies show, several effects of morphine are induced by the dopaminergic
system. Sulpiride is a dopamine D2 receptor (DAD2) antagonist widely used in clinics to treat DArelated
disorders. DAD2 receptors are abundant at hippocampal cornu ammonis (CA1).
Objectives: This study aimed to investigate the possible interaction of morphine and sulpiride on
DA synapses in CA1.
Materials & Methods: In this study, 48 Wistar rats weighing 220 to 250 g were used. These animals
were classified into eight groups (6 rats per group): saline control group (1 ml/kg), morphine
group (5 mg/kg), sulpiride groups alone (1, 2, and 4 mg/kg) and sulpiride groups (1, 2, and 4 mg/
kg)+morphine (5 mg/kg). Saline or substances were injected once intraperitoneally. After 48 h, the
animals’ brains were removed under anesthesia and placed in 10% formalin for fixation. Then, 3- to
4-μm slices were cut from these tissues, and the DA synapse was examined by histochemistry and
immunohistochemistry techniques. The data were statistically analyzed by the analysis of variance.
Results: The control group had DA synapses and healthy neurons. A relative increase in DA
synapses compared to the control group was observed in the morphine and single sulpiride groups.
However, in sulpiride+morphine groups, DA synapses were reduced compared to morphine or
sulpiride alone, but neurons were not destroyed.
Conclusion: The interaction effect of sulpiride and morphine in the CA1 region may decrease DA
synapses.
system. Sulpiride is a dopamine D2 receptor (DAD2) antagonist widely used in clinics to treat DArelated
disorders. DAD2 receptors are abundant at hippocampal cornu ammonis (CA1).
Objectives: This study aimed to investigate the possible interaction of morphine and sulpiride on
DA synapses in CA1.
Materials & Methods: In this study, 48 Wistar rats weighing 220 to 250 g were used. These animals
were classified into eight groups (6 rats per group): saline control group (1 ml/kg), morphine
group (5 mg/kg), sulpiride groups alone (1, 2, and 4 mg/kg) and sulpiride groups (1, 2, and 4 mg/
kg)+morphine (5 mg/kg). Saline or substances were injected once intraperitoneally. After 48 h, the
animals’ brains were removed under anesthesia and placed in 10% formalin for fixation. Then, 3- to
4-μm slices were cut from these tissues, and the DA synapse was examined by histochemistry and
immunohistochemistry techniques. The data were statistically analyzed by the analysis of variance.
Results: The control group had DA synapses and healthy neurons. A relative increase in DA
synapses compared to the control group was observed in the morphine and single sulpiride groups.
However, in sulpiride+morphine groups, DA synapses were reduced compared to morphine or
sulpiride alone, but neurons were not destroyed.
Conclusion: The interaction effect of sulpiride and morphine in the CA1 region may decrease DA
synapses.
Type of Study: Research |
Subject:
Special
Received: 2022/09/14 | Accepted: 2022/09/28 | Published: 2022/09/28
Received: 2022/09/14 | Accepted: 2022/09/28 | Published: 2022/09/28
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