Volume 7, Issue 3 (Summer 2021)
Caspian J Neurol Sci 2021, 7(3): 172-179 |
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Mojtaba Hedayati Ch1 
, Mahmood Abedinzade * 2, Korosh Khanaki3 , Behrooz Khakpour Tleghani4 , Mostafa Golshekan5 , Ekram Mohammadi6
, Mahmood Abedinzade * 2, Korosh Khanaki3 , Behrooz Khakpour Tleghani4 , Mostafa Golshekan5 , Ekram Mohammadi6
1- Department of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
2- Department of Physiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran. ,mabedinzade@gums.ac.ir
3- Department of Clinical Biochemistry, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran.
4- Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
5- Medical Biotechnology Research Center, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran.
6- Department of Medical Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
2- Department of Physiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran. ,
3- Department of Clinical Biochemistry, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran.
4- Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
5- Medical Biotechnology Research Center, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran.
6- Department of Medical Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Abstract: (1387 Views)
Background: Pathological conditions, including ischemic stroke, are associated with severe Endoplasmic Reticulum (ER) stress that induces apoptosis and cell death. Herbal medicines are natural treatments with few side effects in such situations.
Objectives: In the present study, we examined the probable neuroprotective effects of Viola spathulata, Lamium album, and Urtica dioica on splicing of ER stress mRNA gene marker (X-box Binding Protein-1 [XBP-1]) and Unfolded Protein Response (UPR) activation in the brain of the rat stroke model.
Materials & Methods: Sixty male Wistar albino rats (weight: 220-250 g) were selected for this study and divided into two major groups. The first major group comprised the healthy animals that are subdivided into groups number 1 to 5. The second major group comprised the Middle Cerebral Artery Occlusion (MCAO) group subdivided into groups number 6-10. The reverse transcriptionpolymerase chain reaction was performed after pretreatment with different herbal extracts (5, 10 mg/kg), Viola spathulata, and 100 mg/ kg Urtica dioica and Lamium album). The results were analyzed by one-way analysis of variance in IBM SPSS v. 22.
Results: MCAO-induced ischemia caused a marked increase in XBP-1 splicing in all rats of the MCAO group in comparison to the control groups (P<0.05), and pretreatment with 3 herbal extracts dramatically decreased target gene splicing in the MCAO studied groups (P<0.05).
Conclusion: All three herbal extracts of U. dioica, L. album, and V. spathulata had the promising potential to use as a neuroprotective agent by reducing ER stress.
Objectives: In the present study, we examined the probable neuroprotective effects of Viola spathulata, Lamium album, and Urtica dioica on splicing of ER stress mRNA gene marker (X-box Binding Protein-1 [XBP-1]) and Unfolded Protein Response (UPR) activation in the brain of the rat stroke model.
Materials & Methods: Sixty male Wistar albino rats (weight: 220-250 g) were selected for this study and divided into two major groups. The first major group comprised the healthy animals that are subdivided into groups number 1 to 5. The second major group comprised the Middle Cerebral Artery Occlusion (MCAO) group subdivided into groups number 6-10. The reverse transcriptionpolymerase chain reaction was performed after pretreatment with different herbal extracts (5, 10 mg/kg), Viola spathulata, and 100 mg/ kg Urtica dioica and Lamium album). The results were analyzed by one-way analysis of variance in IBM SPSS v. 22.
Results: MCAO-induced ischemia caused a marked increase in XBP-1 splicing in all rats of the MCAO group in comparison to the control groups (P<0.05), and pretreatment with 3 herbal extracts dramatically decreased target gene splicing in the MCAO studied groups (P<0.05).
Conclusion: All three herbal extracts of U. dioica, L. album, and V. spathulata had the promising potential to use as a neuroprotective agent by reducing ER stress.
Type of Study: Research |
Subject:
Special
Received: 2021/08/25 | Accepted: 2021/07/7 | Published: 2021/07/7
Received: 2021/08/25 | Accepted: 2021/07/7 | Published: 2021/07/7
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