Volume 5, Issue 4 (Autumn 2019)
Caspian J Neurol Sci 2019, 5(4): 161-167 |
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Marzieh Zamani1 
, Kambiz Rohampour * 1, Samira Rashtiani2 , Masoume Dolati3 , Faranak Fallahian3 , Naser Kalhor4
, Kambiz Rohampour * 1, Samira Rashtiani2 , Masoume Dolati3 , Faranak Fallahian3 , Naser Kalhor4
1- Neuroscience Research Center, Department of Physiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
2- Student Research Committee, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
3- Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
4- Highly Specialized Jihad Daneshgahi Infertility Center, Qom University of Medical Sciences, Qom, Iran
2- Student Research Committee, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
3- Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
4- Highly Specialized Jihad Daneshgahi Infertility Center, Qom University of Medical Sciences, Qom, Iran
Abstract: (2437 Views)
Background: Type 2 diabetes mellitus (T2DM) is one of the significant risk factors for Alzheimer disease (AD). Defects in insulin signaling pathway induce AD hallmarks mainly through activation of glycogen synthase kinase-3β (GSK-3β) pathway.
Objectives: In this study, we investigated the expression of GSK-3β and insulin receptor (IR) genes in the hippocampi of an animal model of sporadic AD and assessed the preventive effect of Catechin (CAT) and epigallocatechin gallate (EGCG) on their expression.
Materials & Methods: Adult male Wistar rats were treated by intracerebroventricular streptozotocin (STZ) injection (3 mg/kg) at day 1 and 3 after cannulation. CAT was administered at a dose of 40 mg/kg for 10 days per gavage, and EGCG was administered at a dose of 3 mg/kg for 14 days into drinking water. Then the animals were decapitated, and their hippocampi were removed. Real-time Polymerase Chain Reaction (PCR) was used to evaluate the alteration in gene expression.
Results: There was overexpression in GSK-3β gene in STZ-treated rats (P≤0.05), which was brought back to normalcy by EGCG (P≤0.01). The IR gene also increased after STZ treatment, but CAT reduced IR expression (P≤0.05). However, the suppressive effect of EGCG on IR expression was stronger (P≤0.01).
Conclusion: The neuroprotective activity of EGCG might be due to its influence on IR and GSK-3β expression.
Objectives: In this study, we investigated the expression of GSK-3β and insulin receptor (IR) genes in the hippocampi of an animal model of sporadic AD and assessed the preventive effect of Catechin (CAT) and epigallocatechin gallate (EGCG) on their expression.
Materials & Methods: Adult male Wistar rats were treated by intracerebroventricular streptozotocin (STZ) injection (3 mg/kg) at day 1 and 3 after cannulation. CAT was administered at a dose of 40 mg/kg for 10 days per gavage, and EGCG was administered at a dose of 3 mg/kg for 14 days into drinking water. Then the animals were decapitated, and their hippocampi were removed. Real-time Polymerase Chain Reaction (PCR) was used to evaluate the alteration in gene expression.
Results: There was overexpression in GSK-3β gene in STZ-treated rats (P≤0.05), which was brought back to normalcy by EGCG (P≤0.01). The IR gene also increased after STZ treatment, but CAT reduced IR expression (P≤0.05). However, the suppressive effect of EGCG on IR expression was stronger (P≤0.01).
Conclusion: The neuroprotective activity of EGCG might be due to its influence on IR and GSK-3β expression.
Type of Study: Research |
Subject:
Special
Received: 2019/03/12 | Accepted: 2019/08/8 | Published: 2019/10/1
Received: 2019/03/12 | Accepted: 2019/08/8 | Published: 2019/10/1
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