Volume 7, Issue 1 (Winter 2021)                   Caspian.J.Neurol.Sci 2021, 7(1): 42-50 | Back to browse issues page

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1- Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran
Abstract:   (176 Views)
Background: Ischemia causes severe neuronal damage and induces oxidative stress, memory
impairment, and reduces pain threshold. Herniarin is a powerful antioxidant.
Objectives: This study aimed to evaluate the effect of herniarin on memory, pain, and oxidative
stress in an ischemia model in male rats.
Materials & Methods: In this study, 50 male rats were divided into 5 groups of control, sham,
ischemic, and two other ischemic groups, which received herniarin at doses of 150 and 300 mg/
kg by gavage for 14 days. Behavioral tests were performed by shuttle box, and Y-maze and pain
tests were performed by Tail-Flick test. Then, the rats’ brains were extracted to evaluate lipid
peroxidation and measure the levels of thiol and Glutathione Peroxidase (GPX) in the hippocampus
and striatum tissues. The results were expressed as Mean±SEM and then analyzed using suitable
statistical methods of ANOVA and least significant difference post-hoc test in
SPSS V. 20.
Results: Herniarin significantly increased the avoidance memory, spatial memory, and pain
thresholds of ischemic rats at different concentrations (P<0.001). Besides, the amount of
malondialdehyde (MDA) and thiol in the ischemic group increased significantly in comparison to
the control group (P<0.001). Also, in the ischemic group, GPX (P<0.001) significantly decreased.
Decreased MDA (P<0.001) and thiol (P<0.001) and increased GPX levels were observed with
herniarin administration (P<0.01).
Conclusion: According to this study’s results, herniarin can remove free radicals and oxidant
substances from the brain. Thus, it improves memory and pain thresholds in the brain hypoperfusion
ischemia model.
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Type of Study: Research | Subject: Special
Received: 2021/03/17 | Accepted: 2021/01/21 | Published: 2021/01/21