Volume 7, Issue 1 (Winter 2021)                   Caspian.J.Neurol.Sci 2021, 7(1): 1-9 | Back to browse issues page

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1- Student Research Committee, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
2- Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
3- Neuroscience Research Center, Guilan University of Medical Sciences, Rasht, Iran
4- Neurology Department, Poursina Hospital, Guilan University of Medical Sciences, Rasht, Iran
Abstract:   (163 Views)
Background: Alzheimer Disease (AD) is an age-related neurodegenerative disorder with a
progressive impairment of cognitive function. The pineal gland hormone melatonin (MEL) has
been known as a protection agent against AD. However, the effect of melatonin in various doses is
Objectives: In this study, we aimed to investigate two doses of MEL on learning and memory in the
amyloid-βeta (Aβ)-induced AD in the rats.
Materials & Methods: Forty-eight male Wistar rats were used in the experiment and randomly
divided control, sham, vehicle, AD, AD+MEL10 mg/kg, and AD+MEL 20 mg/kg groups.
Intracerebroventricular injection of Aβ1–42 was used to develop the animal model of AD. Also,
MEL-treated groups received an intraperitoneal injection of MEL for 4 next weeks. The Morris
Water Maze (MWM) and Passive Avoidance Learning (PAL) tests were used to examine animals’
learning and memory. The brain of animals was removed for immunohistochemistry for anti-
Amyloid Precursor Protein (APP).
Results: Intra-peritoneal injection of MEL significantly improve learning and memory in MWM
(P=0.000) and PAL test (P=0.000), but there were no significant changes in the two groups that
received the melatonin (P>0.05). Histopathological analysis revealed that the clearance of APP
deposition in the AD+MEL20 group was considerable compared with the AD+MEL10 group
Conclusion: Our findings indicate that 10 and 20 mg/kg doses of melatonin have similar results on
learning and memory in the AD model. But 20 mg/kg of melatonin has significantly more effect on
the clearance of APP deposition.
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Type of Study: Research | Subject: Special
Received: 2021/03/14 | Accepted: 2021/01/21 | Published: 2021/01/21