[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 2, Number 6 (Summer 2016) ::
Caspian.J.Neurol.Sci 2016, 2(6): 1-9 Back to browse issues page
Effects of Green Tea Epigallocatechin-3-Gallate (EGCG) On Proteolipid Protein (PLP) and Oligodendrocyte Transcription Factor 1 (Olig1) Expression in the Cerebral Cortex of Cuprizone Induced Multiple Sclerosis Mice; A Western Blot Study
Mohammad-Reza Semnani, Farhad Mashayekhi, Mahnaz Azarnia , Zivar Salehi
Department of Cell and Molecular Biology, School of Biological Sciences, Kharazmi University, Tehran, Iran; Azarnia.mahnaz1957@gmail.com
Abstract:   (2161 Views)

Background: The cuprizone multiple sclerosis (MS) animal model is characteristic for toxic demyelination and represents a reversible demyelination and remyelination system. It has been shown that green tea epigallocatechin-3-gallate (EGCG) might be effective in improving the symptoms and pathological conditions associated with autoimmune inflammatory diseases in several animal models.

Objectives: In this study the effects of EGCG on proteolipid protein (PLP) and oligodendrocyte transcription factor 1 (Olig1) expression in the cerebral cortex of murine model of cuprizone-induced demyelination was investigated.

Materials and Methods: C57BL/6 mice were treated by cuprizone for six weeks in order to induce demyelination. Immediately after the cessation of cuprizone the animals were divided into 6 groups (n=10 for each groups). The first two groups was injected intraperitoneally (IP) by EGCG in the amount of 50 mg/kg /daily body weight for 2 and 4 weeks. The second two groups (SHAM) was injected IP by phosphate-buffered saline (PBS) for 2 and 4 weeks and the third two groups was left without injection as controls. After two and four weeks the mice were killed and the cerebral cortex was collected and the expression of PLP and Olig1 was studied by Western blotting.

Results:The results showed that there is a significant increases in PLP and Olig1 expression among the EGCG treated group as compared to the SHAM and control groups (p<0.0001).

Conclusion: It is concluded that EGCG increases PLP and Olig1 expression in the cerebral cortex of mouse model of MS induced by cuprizone.

Keywords: Cuprizone, Multiple Sclerosis, Encephalomyelitis, Autoimmune, Experimental, Epigallocatechin-3-gallate, Myelin Proteolipid Protein, Oligodendrocyte Transcription Factor 1
Full-Text [PDF 215 kb]   (1105 Downloads) |   |   Full-Text (HTML)  (348 Views)  
Type of Study: Research | Subject: Special
Add your comments about this article
Your username or email:

Write the security code in the box >



DOI: 10.18869/acadpub.cjns.2.6.1


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Semnani M, Mashayekhi F, Azarnia M, Salehi Z. Effects of Green Tea Epigallocatechin-3-Gallate (EGCG) On Proteolipid Protein (PLP) and Oligodendrocyte Transcription Factor 1 (Olig1) Expression in the Cerebral Cortex of Cuprizone Induced Multiple Sclerosis Mice; A Western Blot Study . Caspian.J.Neurol.Sci. 2016; 2 (6) :1-9
URL: http://cjns.gums.ac.ir/article-1-112-en.html
Volume 2, Number 6 (Summer 2016) Back to browse issues page
مجله علوم اعصاب کاسپین Caspian Journal of Neurological Sciences

   The journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

Persian site map - English site map - Created in 0.893 seconds with 815 queries by yektaweb 3455